FG11F-C-FLAG-hNKD2
(Plasmid
#174330)
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Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFG11F
- Total vector size (bp) 9981
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNone
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHomo sapiens NKD inhibitor of WNT signaling pathway 2
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Alt nameNKD2
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SpeciesH. sapiens (human)
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Insert Size (bp)1355
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GenBank IDNM_033120.4
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Entrez GeneNKD2 (a.k.a. Naked2)
- Promoter UbC
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Tag
/ Fusion Protein
- FLAG Tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer UbC-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FG11F-C-FLAG-hNKD2 was a gift from Sonal Srikanth (Addgene plasmid # 174330 ; http://n2t.net/addgene:174330 ; RRID:Addgene_174330) -
For your References section:
NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca(2+) entry in T cells. Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y. Cell Rep. 2021 Aug 24;36(8):109603. doi: 10.1016/j.celrep.2021.109603. 10.1016/j.celrep.2021.109603 PubMed 34433025