pWZL hygro Flag HA TRAP220 wt
(Plasmid #17433)

• Purpose: Retroviral expression of Med1/TRAP220 in mammalian cells
• Depositing Lab: Kai Ge
• Publication: Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

Backbone

• Vector backbone: pWZL Hygro
• Backbone size w/o insert (bp): 5800
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRAP220
• Alt name: Med1
• Species: H. sapiens (human)
• Insert Size (bp): 4700
• GenBank ID: NM_004774
• Entrez Gene: MED1 (a.k.a. CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2)
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBabe 5'

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry