Skip to main content
Addgene

pLentiguide-puro_hORAI1sg
(Plasmid #174326)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174326 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLentiguide Puro
  • Backbone size w/o insert (bp) 10183
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Orai1
  • gRNA/shRNA sequence
    G ATC GGC CAG AGT TAC TCC G
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • GenBank ID
    NM_032790.3 NM_032790.3
  • Entrez Gene
    ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmB1 (not destroyed)
  • 3′ cloning site BsmB1 (not destroyed)
  • 5′ sequencing primer hU6-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiguide-puro_hORAI1sg was a gift from Sonal Srikanth (Addgene plasmid # 174326 ; http://n2t.net/addgene:174326 ; RRID:Addgene_174326)

  • For your References section:

    NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca(2+) entry in T cells. Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y. Cell Rep. 2021 Aug 24;36(8):109603. doi: 10.1016/j.celrep.2021.109603. 10.1016/j.celrep.2021.109603 PubMed 34433025
Related items:
Commonly requested with: