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Addgene

pMal-SLFN2
(Plasmid #174322)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174322 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMal
  • Backbone manufacturer
    NEB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SLFN2
  • Alt name
    Shl
  • Alt name
    Shlf2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1137
  • Entrez Gene
    Slfn2 (a.k.a. Shlf2)
  • Promoter tac promoter
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcorI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer GATGAAGCCCTGAAAGACGCGCAG
  • 3′ sequencing primer GCGGGCCTCTTCGCTATTAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMal-SLFN2 was a gift from Bruce Beutler (Addgene plasmid # 174322 ; http://n2t.net/addgene:174322 ; RRID:Addgene_174322)

  • For your References section:

    SLFN2 protection of tRNAs from stress-induced cleavage is essential for T cell-mediated immunity. Yue T, Zhan X, Zhang D, Jain R, Wang KW, Choi JH, Misawa T, Su L, Quan J, Hildebrand S, Xu D, Li X, Turer E, Sun L, Moresco EMY, Beutler B. Science. 2021 May 14;372(6543). pii: 372/6543/eaba4220. doi: 10.1126/science.aba4220. 10.1126/science.aba4220 PubMed 33986151
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