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Addgene

pDGO186N-KS3
(Plasmid #174303)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDGO143
  • Backbone size w/o insert (bp) 3587
  • Total vector size (bp) 4423
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    spacer expression cassette
  • gRNA/shRNA sequence
    AGAATCGCACGGTAAAGCTCTCCCGCTTCACACGCAC
  • Species
    Clostridium Thermocellum

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCTTGATGACACAGAAGAAGGCGATTTGA
  • 3′ sequencing primer TTGATATGCCTCCTAAATTTTTATCTAAAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDGO186N-KS3 was a gift from Lee Lynd (Addgene plasmid # 174303 ; http://n2t.net/addgene:174303 ; RRID:Addgene_174303)

  • For your References section:

    A Single Nucleotide Change in the polC DNA Polymerase III in Clostridium thermocellum Is Sufficient To Create a Hypermutator Phenotype. Lanahan A, Zakowicz K, Tian L, Olson DG, Lynd LR. Appl Environ Microbiol. 2022 Jan 11;88(1):e0153121. doi: 10.1128/AEM.01531-21. Epub 2021 Oct 20. 10.1128/AEM.01531-21 PubMed 35015978
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