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Liu Lab MusCK Library
(Pooled Libraries #174196, #174197, #1000000178)

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  • Purpose

    This lentiviral murine CRISPR-Cas9 knockout (MusCK) library contains 49,252 guide RNAs targeting 4,922 mouse genes implicated in tumor initiation, progression, and immune modulation and 1,000 control gRNAs in each half-library. The library is divided into 2 parts (MusCK Library A and MusCK Library B). The A and B libraries contain in total 10 gRNAs per gene (5 gRNAs in each half-library). Researchers only need an individual library for an experiment.

  • Vector Backbone

    lentiCRISPR v2 (Plasmid #52961) - expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000178 Two tubes, each with a half-library (Library A and B) in backbone lentiCRISPR v2
  • #174196 (Library A)
  • #174197 (Library B)
 1 $750 Add to Cart
Pooled Library 174196 Library A, one tube, contains Liu Lab MusCK Library A 1 $540 Add to Cart
Pooled Library 174197 Library B, one tube, contains Liu Lab MusCK Library B 1 $540 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Mouse
  • Genes targeted
    4,922
  • gRNAs
    24,622 (Library A), 25,630 (Library B)
  • Controls
    2,000 control gRNAs (1,000 in each half-library)
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

The depositing lab recommends using MAGeCK algorithms for analysis.

Please visit https://doi.org/10.1101/2020.12.09.418012 for bioRxiv preprint.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Liu Lab MusCK Libraries A and B were a gift from Xiaole Shirley Liu (Addgene #1000000178)
    Liu Lab MusCK Libraries A was a gift from Xiaole Shirley Liu (Addgene #174196)
    Liu Lab MusCK Libraries B was a gift from Xiaole Shirley Liu (Addgene #174197)

  • For your References section:

    In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target. Wang X, Tokheim C, Gu SS, Wang B, Tang Q, Li Y, Traugh N, Zeng Z, Zhang Y, Li Z, Zhang B, Fu J, Xiao T, Li W, Meyer CA, Chu J, Jiang P, Cejas P, Lim K, Long H, Brown M, Liu XS. Cell. 2021 Oct 14;184(21):5357-5374.e22. doi: 10.1016/j.cell.2021.09.006. PMID: 34582788.
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