pcDNA-Stra6L-6His
(Plasmid
#174215)
-
PurposeStra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 174215 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1+N-6His
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5514
- Total vector size (bp) 7348
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameStra6L
-
Alt nameRbpr2
-
Alt nameRb
-
Alt name1300002K09Rik
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1880
-
Mutationnon
-
GenBank IDNM_001305421.1 74152
-
Entrez GeneStra6l (a.k.a. 1300002K09Rik, Rb, Rbpr2)
- Promoter CMV
-
Tag
/ Fusion Protein
- 6-His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV
- 3′ sequencing primer SV40 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byCustom gene synthesis from GenScript.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA-Stra6L-6His was a gift from Adrian Press (Addgene plasmid # 174215 ; http://n2t.net/addgene:174215 ; RRID:Addgene_174215) -
For your References section:
Poly(2-oxazoline) Homopolymers and Diblock Copolymers Containing Retinoate ω-End Groups. Stafast LM, Weber C, Kuchenbrod MT, Hoeppener S, Behnke M, Schubert S, Mehmetaj K, Press AT, Bauer M, Schubert US. ACS Appl. Polym. Mater. 2022 10.1021/acsapm.2c00037