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Addgene

pTN4
(Plasmid #174212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Vector type
    Worm Expression, Cre/Lox, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    AtTIR1(F79G)::mRuby
  • Species
    C. elegans (nematode)
  • Entrez Gene
    TIR1 (a.k.a. AT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1)
  • Tag / Fusion Protein
    • mRuby (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    GFP
  • Species
    C. elegans (nematode)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTN4 was a gift from Masato Kanemaki (Addgene plasmid # 174212 ; http://n2t.net/addgene:174212 ; RRID:Addgene_174212)

  • For your References section:

    The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans. Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa H, Hayashi KI, Harata M, Kanemaki MT. Genetics. 2021 Dec 2. pii: 6448232. doi: 10.1093/genetics/iyab218. 10.1093/genetics/iyab218 PubMed 34865044
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