p406MET25
(Plasmid
#17421)
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep406ADH1
- Backbone size w/o insert (bp) 2691
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMET25 promoter + polylinker + CYC1 terminator
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Alt nameMET17 promoter (official name in SGD)
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Alt nameMET15 promoter
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Alt nameO-acetyl homoserine-O-acetyl serine sulfhydrylase
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2301
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MutationMET25 promoter is between SacI and XbaI restriction sites.
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Entrez GeneMET17 (a.k.a. YLR303W, MET15, MET25)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglI (not destroyed)
- 3′ cloning site BglI (not destroyed)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPlasmid was created by Paula Calle. Insert was cut from Mumberg plasmid p425MET25, which was obtained from ATCC.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p406MET25 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 17421 ; http://n2t.net/addgene:17421 ; RRID:Addgene_17421)