p404MET25
(Plasmid #17420)

• Depositing Labs: Nicolas Buchler, Fred Cross
• Publication: Buchler Lab plasmids (unpublished)

Backbone

• Vector backbone: p404ADH1
• Backbone size w/o insert (bp): 2581
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MET25 promoter + polylinker + CYC1 terminator
• Alt name: MET17 promoter (official name in SGD)
• Alt name: MET15 promoter
• Alt name: O-acetyl homoserine-O-acetyl serine sulfhydrylase
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2301
• Mutation: MET25 promoter is between SacI and XbaI restriction sites.
• Entrez Gene: MET17 (a.k.a. YLR303W, MET15, MET25)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglI (not destroyed)
• 3′ cloning site: BglI (not destroyed)
• 5′ sequencing primer: M13 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid was created by Paula Calle. Insert was cut from Mumberg plasmid p425MET25, which was obtained from ATCC.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).