Dok1-EGFP
(Plasmid
#174194)
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PurposeExpresses Dok1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 174194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDest-eGFP-N1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDOK1
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SpeciesH. sapiens (human)
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GenBank IDBC114440.1
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Entrez GeneDOK1 (a.k.a. P62DOK, pp62)
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dok1-EGFP was a gift from Cheng-han Yu (Addgene plasmid # 174194 ; http://n2t.net/addgene:174194 ; RRID:Addgene_174194) -
For your References section:
Integrin-beta3 clusters recruit clathrin-mediated endocytic machinery in the absence of traction force. Yu CH, Rafiq NB, Cao F, Zhou Y, Krishnasamy A, Biswas KH, Ravasio A, Chen Z, Wang YH, Kawauchi K, Jones GE, Sheetz MP. Nat Commun. 2015 Oct 28;6:8672. doi: 10.1038/ncomms9672. 10.1038/ncomms9672 PubMed 26507506