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Addgene

p404MET3
(Plasmid #17419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17419 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p404ADH1
  • Backbone size w/o insert (bp) 2581
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MET3 promoter + polylinker + CYC1 terminator
  • Alt name
    ATP sulfurylase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2413
  • Mutation
    MET3 promoter is between SacI and XbaI restriction sites.
  • Entrez Gene
    MET3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglI (not destroyed)
  • 3′ cloning site BglI (not destroyed)
  • 5′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid was created by Paula Calle. Insert was cut from p405MET3.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p404MET3 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 17419 ; http://n2t.net/addgene:17419 ; RRID:Addgene_17419)
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