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Addgene

pLenti-AncBE4max
(Plasmid #174164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Transfer plasmid of the 3rd generation lentiviral system
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AncBE4max
  • Tag / Fusion Protein
    • P2A-mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-AncBE4max was a gift from Wensheng Wei (Addgene plasmid # 174164 ; http://n2t.net/addgene:174164 ; RRID:Addgene_174164)

  • For your References section:

    Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs. Xu P, Liu Z, Liu Y, Ma H, Xu Y, Bao Y, Zhu S, Cao Z, Wu Z, Zhou Z, Wei W. Nat Biotechnol. 2021 Jun 21. pii: 10.1038/s41587-021-00944-1. doi: 10.1038/s41587-021-00944-1. 10.1038/s41587-021-00944-1 PubMed 34155407
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