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Addgene

pLenti6.2-coHPDL H258A 3xFlag-V5
(Plasmid #174130)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174130 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.2
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    4-hydroxyphenylpyruvate dioxygenase like
  • Species
    H. sapiens (human)
  • Mutation
    Codon optimized, H258A
  • GenBank ID
    NM_032756.4
  • Entrez Gene
    HPDL (a.k.a. 4-HPPD-L, GLOXD1, NEDSWMA, SPG83)
  • Tag / Fusion Protein
    • 3xFLAG-V5 (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.2-coHPDL H258A 3xFlag-V5 was a gift from Michael Pacold (Addgene plasmid # 174130 ; http://n2t.net/addgene:174130 ; RRID:Addgene_174130)

  • For your References section:

    The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 synthesis pathway. Banh RS, Kim ES, Spillier Q, Biancur DE, Yamamoto K, Sohn ASW, Shi G, Jones DR, Kimmelman AC, Pacold ME. Nature. 2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 10.1038/s41586-021-03865-w. 10.1038/s41586-021-03865-w PubMed 34471290
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