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Addgene

pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro
(Plasmid #174118)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174118 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
  • gRNA/shRNA sequence
    TGATATCACAG
  • Promoter mU6 and UbC

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro was a gift from Stanley Qi (Addgene plasmid # 174118 ; http://n2t.net/addgene:174118 ; RRID:Addgene_174118)

  • For your References section:

    Interrogation of the dynamic properties of higher-order heterochromatin using CRISPR-dCas9. Gao Y, Han M, Shang S, Wang H, Qi LS. Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 10.1016/j.molcel.2021.07.034. 10.1016/j.molcel.2021.07.034 PubMed 34428454
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