pBabe zeo HMGA2
(Plasmid #17411)

• Depositing Lab: Tyler Jacks
• Publication: Kumar et al Proc Natl Acad Sci U S A. 2008 Feb 28. ():.

Backbone

• Vector backbone: pBabe-Zeo
• Backbone manufacturer: Available at Addgene (#1766)
• Backbone size w/o insert (bp): 4900
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HMGA2
• Alt name: high mobility group AT-hook 2
• Species: M. musculus (mouse)
• Insert Size (bp): 330
• Entrez Gene: Hmga2 (a.k.a. 9430083A20Rik, HMGI-C, Hmgic, pg, pygmy)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: pBabe 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HMGA2 was a kind gift of C. Mayr
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The ORF of human HMGA2 was amplified using HMGA2F (5’-TAAGGATCCATGAGCGCACGCGGTGAG-3’) and HMGA2R (5’- GCGGAATTCCTAATCCTCCTCTGCGGACTCTTG-3’). The product was cut with BamHI and EcoRI and cloned into pBabe.Zeo.