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Addgene

pAcBac1.tR4-AcdRS82
(Plasmid #174101)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 174101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcBac1
  • Backbone size w/o insert (bp) 9091
  • Total vector size (bp) 10350
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M. barkeri tRNA synthetase for acridone82
  • Species
    Methanosarcina barkeri
  • Insert Size (bp)
    1259
  • Mutation
    N311S, C313G, V366A, W382T
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcBac1.tR4-AcdRS82 was a gift from Ryan Mehl (Addgene plasmid # 174101 ; http://n2t.net/addgene:174101 ; RRID:Addgene_174101)

  • For your References section:

    Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells. Jones CM, Robkis DM, Blizzard RJ, Munari M, Venkatesh Y, Mihaila TS, Eddins AJ, Mehl RA, Zagotta WN, Gordon SE, Petersson EJ. Chem Sci. 2021 Aug 3;12(36):11955-11964. doi: 10.1039/d1sc01914g. eCollection 2021 Sep 22. 10.1039/d1sc01914g PubMed 34976337
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