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Addgene

pDule2-Acd82
(Plasmid #174100)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174100 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDule2
  • Backbone size w/o insert (bp) 4474
  • Total vector size (bp) 5734
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Acridone 82 tRNA synthetase and tRNA
  • Species
    Methanosarcina barkeri
  • Insert Size (bp)
    1260
  • Mutation
    N311S, C313G, V366A, W382T

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDule2-Acd82 was a gift from Ryan Mehl (Addgene plasmid # 174100 ; http://n2t.net/addgene:174100 ; RRID:Addgene_174100)

  • For your References section:

    Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells. Jones CM, Robkis DM, Blizzard RJ, Munari M, Venkatesh Y, Mihaila TS, Eddins AJ, Mehl RA, Zagotta WN, Gordon SE, Petersson EJ. Chem Sci. 2021 Aug 3;12(36):11955-11964. doi: 10.1039/d1sc01914g. eCollection 2021 Sep 22. 10.1039/d1sc01914g PubMed 34976337
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