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Addgene

hs-calx2
(Plasmid #1741)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1741 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaspeRhs
  • Backbone size w/o insert (bp) 8780
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    calx2
  • Alt name
    Na/Ca Exchange Protein
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2860
  • Mutation
    N/A
  • GenBank ID
    NM_169940
  • Entrez Gene
    Calx (a.k.a. Dmel_CG5685, 9C5, CALX, CALX1.1, CG5685, CalX, Dmel/Ncx, Dmel\CG5685, NCX, Ncx, anon-93ABa, calX, calx)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer TCCCCGACACCAGCA
  • 3′ sequencing primer CTACTGAAATCTGCC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pBS-calx1.2 ; provided by Dr. Hryshko, Institute of Cardiovascular Sciences, Manitoba, Canada

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hs-calx2 was a gift from Craig Montell (Addgene plasmid # 1741 ; http://n2t.net/addgene:1741 ; RRID:Addgene_1741)

  • For your References section:

    Light Activation, Adaptation, and Cell Survival Functions of the Na(+)/Ca(2+) Exchanger CalX. Wang T, Xu H, Oberwinkler J, Gu Y, Hardie RC, Montell C. Neuron 2005 Feb 3;45(3):367-78. 10.1016/j.neuron.2004.12.046 PubMed 15694324
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