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Addgene

pMH0004
(Plasmid #174087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Total vector size (bp) 14525
  • Vector type
    Lentiviral
  • Selectable markers
    BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas9-BFP
  • Promoter SFFV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer acgggaagcaatagcatgatac
  • 3′ sequencing primer agtgcaggggaaagaatagtagac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See bioRxiv article for preprint info - https://www.biorxiv.org/content/10.1101/775080v2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH0004 was a gift from Jonathan Weissman (Addgene plasmid # 174087 ; http://n2t.net/addgene:174087 ; RRID:Addgene_174087)

  • For your References section:

    Functional single-cell genomics of human cytomegalovirus infection. Hein MY, Weissman JS. Nat Biotechnol. 2022 Mar;40(3):391-401. doi: 10.1038/s41587-021-01059-3. Epub 2021 Oct 25. 10.1038/s41587-021-01059-3 PubMed 34697476
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