Skip to main content
Addgene

pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
(Plasmid #174081)

Print

Ordering

This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 174081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAcBac1
  • Backbone size w/o insert (bp) 9015
  • Total vector size (bp) 10350
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
  • Species
    Methanosarcina barkeri
  • Insert Size (bp)
    1335
  • Mutation
    L270G, Y271L, N311G, C313A
  • Promoter CMV
  • Tag / Fusion Protein
    • NES-Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 was a gift from Ryan Mehl (Addgene plasmid # 174081 ; http://n2t.net/addgene:174081 ; RRID:Addgene_174081)

  • For your References section:

    Access to Faster Eukaryotic Cell Labeling with Encoded Tetrazine Amino Acids. Jang HS, Jana S, Blizzard RJ, Meeuwsen JC, Mehl RA. J Am Chem Soc. 2020 Apr 22;142(16):7245-7249. doi: 10.1021/jacs.9b11520. Epub 2020 Apr 10. 10.1021/jacs.9b11520 PubMed 32251579
Related items:
Commonly requested with: