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Addgene

pDule-Tet3.0
(Plasmid #174080)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174080 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDule
  • Backbone size w/o insert (bp) 3801
  • Total vector size (bp) 5061
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tet3.0 tRNA synthetase and tRNA
  • Species
    Methanosarcina barkeri
  • Insert Size (bp)
    1260
  • Mutation
    L270G, Y271L, N311G, C313A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDule-Tet3.0 was a gift from Ryan Mehl (Addgene plasmid # 174080 ; http://n2t.net/addgene:174080 ; RRID:Addgene_174080)

  • For your References section:

    Access to Faster Eukaryotic Cell Labeling with Encoded Tetrazine Amino Acids. Jang HS, Jana S, Blizzard RJ, Meeuwsen JC, Mehl RA. J Am Chem Soc. 2020 Apr 22;142(16):7245-7249. doi: 10.1021/jacs.9b11520. Epub 2020 Apr 10. 10.1021/jacs.9b11520 PubMed 32251579
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