Puro.Cre empty vector
(Plasmid #17408)

• Purpose: (Empty Backbone)
• Depositing Lab: Tyler Jacks
• Publication: Kumar et al Proc Natl Acad Sci U S A. 2008 Feb 28. ():.

Backbone

• Vector backbone: Puro.Cre
• Backbone manufacturer: Jacks Lab
• Backbone size (bp): 9200
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: MSCV rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: UbC.Luciferase.PGK.Cre was a kind gift of M. Dupage
• Articles Citing this Plasmid:
  • 18 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Puro.Cre was initially generated by amplification of PuroR from MSCV.Puro (Clontech) with PuroF (5’-CGCTGCGCCGAATTCATGACCGAGTACAAGCCCACG-3’) and PuroR (5’-TAAGCGGCCGCTCGAGTCAGGCACCGGGCTTGCGGGTC-3’), digestion with EcoRI and NotI and cloning into UbC.Luciferase.PGK.Cre to generate Puro.Cre.

For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).