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Addgene

pRBC-sfGFP-134/150TAG
(Plasmid #174077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRBC
  • Backbone manufacturer
    Custom
  • Backbone size w/o insert (bp) 5653
  • Total vector size (bp) 6397
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Superfolder GFP
  • Species
    Synthetic
  • Insert Size (bp)
    744
  • Mutation
    D134TAG + N150TAG
  • Promoter T7
  • Tag / Fusion Protein
    • His-6 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRBC-sfGFP-134/150TAG was a gift from Ryan Mehl (Addgene plasmid # 174077 ; http://n2t.net/addgene:174077 ; RRID:Addgene_174077)

  • For your References section:

    A Highly Versatile Expression System for the Production of Multiply Phosphorylated Proteins. Zhu P, Gafken PR, Mehl RA, Cooley RB. ACS Chem Biol. 2019 Jul 19;14(7):1564-1572. doi: 10.1021/acschembio.9b00307. Epub 2019 Jun 17. 10.1021/acschembio.9b00307 PubMed 31243963
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