pRBC-sfGFP-150TAG
(Plasmid #174076)

• Purpose: Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,
• Depositing Lab: Ryan Mehl
• Publication: Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-1572. doi: 10.1021/acschembio.9b00307. Epub 2019 Jun 17.

Backbone

• Vector backbone: pRBC
• Backbone manufacturer: Custom
• Backbone size w/o insert (bp): 5653
• Total vector size (bp): 6397
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Superfolder GFP
• Species: Synthetic
• Insert Size (bp): 744
• Mutation: N150TAG
• Promoter: T7
• Tag / Fusion Protein:
  • His-6 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry