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Addgene

pGWB-cLUC
(Plasmid #174051)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174051 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAMBIA1300
  • Backbone size (bp) 12600
  • Modifications to backbone
    gateway compatible attR1-CmR-ccdB-attR2
  • Vector type
    Plant Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    a gift from Prof. Jian-Min Zhou in Chinese Academy of Science
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
https://academic.oup.com/plphys/article/146/2/323/6107098

Please note there are two mutations in Hygr-E180D and A95S. Depositor confirms this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWB-cLUC was a gift from Alberto Macho (Addgene plasmid # 174051 ; http://n2t.net/addgene:174051 ; RRID:Addgene_174051)

  • For your References section:

    A bacterial effector protein prevents MAPK-mediated phosphorylation of SGT1 to suppress plant immunity. Yu G, Xian L, Xue H, Yu W, Rufian JS, Sang Y, Morcillo RJL, Wang Y, Macho AP. PLoS Pathog. 2020 Sep 25;16(9):e1008933. doi: 10.1371/journal.ppat.1008933. eCollection 2020 Sep. 10.1371/journal.ppat.1008933 PubMed 32976518
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