pGWB-nLUC
(Plasmid #174050)

• Purpose: (Empty Backbone) Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay
• Depositing Lab: Alberto Macho
• Publication: Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933. doi: 10.1371/journal.ppat.1008933. eCollection 2020 Sep.

Backbone

• Vector backbone: pCAMBIA1300
• Backbone size (bp): 13430
• Modifications to backbone: attR1-cmR-ccdB-attR2
• Vector type: Plant Expression, Luciferase
• Tag / Fusion Protein:
  • N-terminal luciferase

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Resource Information

• Supplemental Documents:
  • pGWB-nLUC sequence.gb
• A portion of this plasmid was derived from a plasmid made by: gift from Prof. Jian-Min Zhou group in Chinese Academy of Sciences
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
https://academic.oup.com/plphys/article/146/2/323/6107098

Please note there are two mutations in HygR- E180D and A95S. Depositor confirms these do not affect plasmid function.