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Addgene

Lenti-LucAL
(Plasmid #174049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Luc.Cre empty vector
  • Backbone manufacturer
    Addgene plasmid #20905
  • Total vector size (bp) 10510
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LucAL
  • Alt name
    mutant ALG8 neoantigen
  • Alt name
    mutant LAMA4 neoantigen
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1770
  • Mutation
    ALG8 alanine 506 to threonine; LAMA4 glycine 1254 to valine
  • GenBank ID
    NM_199035.2 NM_010681.4
  • Promoter Human Ubiquitin C
  • Tag / Fusion Protein
    • Luciferase (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGA TTT TGT GCC AGA GTC CTT CG
  • 3′ sequencing primer AGGCCTCGGGATTcctaggAACAGCGGTTT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Visit https://dx.doi.org/10.2139/ssrn.3770111 for the preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-LucAL was a gift from Tyler Jacks (Addgene plasmid # 174049 ; http://n2t.net/addgene:174049 ; RRID:Addgene_174049)

  • For your References section:

    Antigen dominance hierarchies shape TCF1(+) progenitor CD8 T cell phenotypes in tumors. Burger ML, Cruz AM, Crossland GE, Gaglia G, Ritch CC, Blatt SE, Bhutkar A, Canner D, Kienka T, Tavana SZ, Barandiaran AL, Garmilla A, Schenkel JM, Hillman M, de Los Rios Kobara I, Li A, Jaeger AM, Hwang WL, Westcott PMK, Manos MP, Holovatska MM, Hodi FS, Regev A, Santagata S, Jacks T. Cell. 2021 Sep 16;184(19):4996-5014.e26. doi: 10.1016/j.cell.2021.08.020. 10.1016/j.cell.2021.08.020 PubMed 34534464
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