Lenti-LucA
(Plasmid #174047)

• Purpose: Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase
• Depositing Lab: Tyler Jacks
• Publication: Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26. doi: 10.1016/j.cell.2021.08.020.

Backbone

• Vector backbone: Luc.Cre empty vector
• Backbone manufacturer: Addgene plasmid #20905
• Total vector size (bp): 10450
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LucA
• Alt name: mutant ALG8 neoantigen
• Species: M. musculus (mouse)
• Insert Size (bp): 1770
• Mutation: ALG8 alanine 506 to threonine
• GenBank ID: NM_199035.2
• Promoter: Human Ubiquitin C
• Tag / Fusion Protein:
  • Luciferase (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGA TTT TGT GCC AGA GTC CTT CG
• 3′ sequencing primer: AGGCCTCGGGATTcctaggAACAGCGGTTT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Visit https://dx.doi.org/10.2139/ssrn.3770111 for the preprint.