pCDF RBP-FL P53
(Plasmid #174042)

• Purpose: Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)
• Depositing Lab: Stewart Loh
• Publication: Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10.7554/eLife.61487.

Backbone

• Vector backbone: pCDFDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3781
• Total vector size (bp): 5667
• Modifications to backbone: tteRBP gene with 14 amino acid linker (GGAASGGAAGGSSAA) was inserted between SacI and AscI restriction enzyme sites.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For protein expression, please use BL21(DE3).
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: P53 gene
• Alt name: TP53
• Species: H. sapiens (human)
• Insert Size (bp): 1182
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
• Promoter: T7
• Tag / Fusion Protein:
  • Thermoanaerobacter Tencongenesis ribose binding protein with N-terminal histag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7T

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original P53 gene sequence was obtained from the plasmid, PHP53B (purchased from ATCC).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry