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Purpose(Empty Backbone) Prime editing in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174038 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
- Backbone size (bp) 3041
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Vector typeMammalian Expression
- Promoter hU6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSelect pink/purple colonies.
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pU6-tevopreq1-GG-acceptor was a gift from David Liu (Addgene plasmid # 174038 ; http://n2t.net/addgene:174038 ; RRID:Addgene_174038) -
For your References section:
Engineered pegRNAs improve prime editing efficiency. Nelson JW, Randolph PB, Shen SP, Everette KA, Chen PJ, Anzalone AV, An M, Newby GA, Chen JC, Hsu A, Liu DR. Nat Biotechnol. 2021 Oct 4. pii: 10.1038/s41587-021-01039-7. doi: 10.1038/s41587-021-01039-7. 10.1038/s41587-021-01039-7 PubMed 34608327