Skip to main content
Addgene

pDB4946
(Plasmid #174018)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDUAL-41nmt1-Pil1-mCherry
  • Backbone manufacturer
    Li-Lin Du's lab
  • Backbone size w/o insert (bp) 11000
  • Modifications to backbone
    BglII to digest the vector and insert Atg14 into it
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU1, URA4

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Atg14
  • Alt name
    SPAC25A8.02
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1173
  • GenBank ID
    NP_593618.1
  • Entrez Gene
    atg14 (a.k.a. SPOM_SPAC25A8.02)
  • Promoter 41nmt1
  • Tag / Fusion Protein
    • Pil1, mCherry, Atg14 (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGTACAAGggtagatctATGCTGCATGTCAATAAATGC
  • 3′ sequencing primer AAGTGGCGCGCCagatctTCATGAAATAAGCTGATATGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDB4946 was a gift from Li-Lin Du (Addgene plasmid # 174018 ; http://n2t.net/addgene:174018 ; RRID:Addgene_174018)

  • For your References section:

    Visual detection of binary, ternary and quaternary protein interactions in fission yeast using a Pil1 co-tethering assay. Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL. J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. Epub 2021 Oct 12. 10.1242/jcs.258774 PubMed 34499173
Related items: