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Addgene

pDB4658
(Plasmid #174017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174017 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDUAL-41nmt1p-GFP
  • Backbone manufacturer
    Li-Lin Du's lab
  • Backbone size w/o insert (bp) 10000
  • Modifications to backbone
    BglII to digest the vector and gel recycle the long fragment, and insert Atg8 into it
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU1, URA4

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Atg8
  • Alt name
    SPBP8B7.24c
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    366
  • GenBank ID
    NP_596531.1
  • Promoter 41nmt1
  • Tag / Fusion Protein
    • GFP, Atg8 (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer attgtacaaaaGATCTATGCGTTCTCAATTCAAGGAC
  • 3′ sequencing primer GTGGCGCGCCagatctCTAAAAAGGAAACACTGTTCCAAATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDB4658 was a gift from Li-Lin Du (Addgene plasmid # 174017 ; http://n2t.net/addgene:174017 ; RRID:Addgene_174017)

  • For your References section:

    Visual detection of binary, ternary and quaternary protein interactions in fission yeast using a Pil1 co-tethering assay. Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL. J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. Epub 2021 Oct 12. 10.1242/jcs.258774 PubMed 34499173
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