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pRNA2
(Plasmid #174005)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174005 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5023
  • Modifications to backbone
    T7 promoter optimized and multiple cloning sequence heavily modified. Two copies of the human beta-globin 3'UTR added. Ampicillin resistance gene was replaced with Kanamycin.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • GenBank ID
    Cloned from U55762.1
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMVfor
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRNA2 was a gift from Stephen Ikeda (Addgene plasmid # 174005 ; http://n2t.net/addgene:174005 ; RRID:Addgene_174005)

  • For your References section:

    A Simple, Highly Efficient Method for Heterologous Expression in Mammalian Primary Neurons Using Cationic Lipid-mediated mRNA Transfection. Williams DJ, Puhl HL, Ikeda SR. Front Neurosci. 2010 Nov 4;4:181. doi: 10.3389/fnins.2010.00181. eCollection 2010. 10.3389/fnins.2010.00181 PubMed 21267423
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