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pJKW1813
(Plasmid #174003)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174003 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MoClo-YTK
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PpCYP90G4
  • Species
    Paris polyphylla
  • Insert Size (bp)
    1458
  • GenBank ID
    TRINITY_DN46772_c3_g1_i1

Gene/Insert 2

  • Gene/Insert name
    ATR1
  • Alt name
    CYP reductase
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    ATR1 (a.k.a. AT4G24520, AR1, ARABIDOPSIS CYTOCHROME REDUCTASE, F22K18.280, F22K18_280, P450 reductase 1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJKW1813 was a gift from Jing-Ke Weng (Addgene plasmid # 174003 ; http://n2t.net/addgene:174003 ; RRID:Addgene_174003)

  • For your References section:

    Repeated evolution of cytochrome P450-mediated spiroketal steroid biosynthesis in plants. Christ B, Xu C, Xu M, Li FS, Wada N, Mitchell AJ, Han XL, Wen ML, Fujita M, Weng JK. Nat Commun. 2019 Jul 19;10(1):3206. doi: 10.1038/s41467-019-11286-7. 10.1038/s41467-019-11286-7 PubMed 31324795
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