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Addgene

pYPQ265N2
(Plasmid #174001)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174001 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNJB91
  • Total vector size (bp) 8871
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rAPOBEC1-zCas9(D10A)-SpRY-UNG
  • Mutation
    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, S1136W, N1317R, A1322R, R1333P, R1335Q, T1337R
  • Tag / Fusion Protein
    • 3X FLAG, NLS

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ265N2 was a gift from Yiping Qi (Addgene plasmid # 174001 ; http://n2t.net/addgene:174001 ; RRID:Addgene_174001)

  • For your References section:

    Exploring C-To-G Base Editing in Rice, Tomato, and Poplar. Sretenovic S, Liu S, Li G, Cheng Y, Fan T, Xu Y, Zhou J, Zheng X, Coleman G, Zhang Y, Qi Y. Front Genome Ed. 2021 Sep 15;3:756766. doi: 10.3389/fgeed.2021.756766. eCollection 2021. 10.3389/fgeed.2021.756766 PubMed 34713268
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