pENTR4-GFP-C3 (w393-1)
(Plasmid #17397)

• Purpose: Gateway entry vector for N-term eGFP fusion, frame C3
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: pENTR4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3024
• Vector type: Mammalian Expression ; Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10F', 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-C3
• Alt name: enhanced green fluorescent protein
• Insert Size (bp): 716

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco I (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: ENTRforw
• 3′ sequencing primer: ENTRrev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest for 17397
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry