pET28_6xHis-PARP1-CAT-L713F
(Plasmid
#173944)
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PurposeBacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePARP1-CAT-L713F
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SpeciesH. sapiens (human)
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MutationL713F, V762A
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Entrez GenePARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Protein residues 661-1014 (protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28_6xHis-PARP1-CAT-L713F was a gift from Joan Conaway (Addgene plasmid # 173944 ; http://n2t.net/addgene:173944 ; RRID:Addgene_173944) -
For your References section:
Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling. Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW. Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118. 10.1073/pnas.2107277118 PubMed 34465625