pET28_6xHis-PARP1delWGR-L713F
(Plasmid #173941)

• Purpose: Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation
• Depositing Lab: Joan Conaway
• Publication: Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118.

Backbone

• Vector backbone: pET28
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PARP1delWGR-L713F
• Species: H. sapiens (human)
• Mutation: Deletion of residues 521-660; L713F, V762A
• Entrez Gene: PARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • 6xHis-PARP1dWGR-L713F_CDS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protein residues 1-520...661-1014 (protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol)