Skip to main content
Addgene

Hygro-Bem1-mCherry-AID
(Plasmid #173925)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pG23A
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Partial Bem1 C-terminus
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1436
  • Tag / Fusion Protein
    • mCherry- AID (C terminal on backbone)

Gene/Insert 2

  • Gene/Insert name
    hygromycin marker
  • Alt name
    HPHMX4
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1897

Gene/Insert 3

  • Gene/Insert name
    Bem1 downstream sequence
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    600

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Derived from Addgene plasmid 102884.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hygro-Bem1-mCherry-AID was a gift from Liedwij Laan (Addgene plasmid # 173925 ; http://n2t.net/addgene:173925 ; RRID:Addgene_173925)

  • For your References section:

    A tractable physical model for the yeast polarity predicts epistasis and fitness. Daalman WK, Sweep E, Laan L. Philos Trans R Soc Lond B Biol Sci. 2023 May 22;378(1877):20220044. doi: 10.1098/rstb.2022.0044. Epub 2023 Apr 3. 10.1098/rstb.2022.0044 PubMed 37004720
Related items: