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Addgene

BPK1520_puroR
(Plasmid #173901)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173901 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    BPK1520
  • Backbone manufacturer
    Joung Lab (Addgene Plasmid #65777)
  • Backbone size (bp) 3636
  • Modifications to backbone
    CMV-driven puromycin resistance cassette
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter CMV/U6
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Puromycin resistance cassette was cloned from PX459 V2.0 pSpCas9 (BB)-2A-Puro (Addgene Plasmid #62988). CMV promoter was cloned from pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA (Addgene Plasmid #61591)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BPK1520_puroR was a gift from Ronald Cohn (Addgene plasmid # 173901 ; http://n2t.net/addgene:173901 ; RRID:Addgene_173901)
  • For your References section:

    Modeling Niemann-Pick disease type C in a human haploid cell line allows for patient variant characterization and clinical interpretation. Erwood S, Brewer RA, Bily TMI, Maino E, Zhou L, Cohn RD, Ivakine EA. Genome Res. 2019 Dec;29(12):2010-2019. doi: 10.1101/gr.250720.119. Epub 2019 Nov 21. 10.1101/gr.250720.119 PubMed 31754021