pLenti X2 Neo DEST (w18-1)
(Plasmid #17390)

• Purpose: (Empty Backbone) 3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size (bp): 9249
• Vector type: Lentiviral, RNAi ; Destination vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Also resistant to Chloramphinecol, DB3.1 or ccDB survival, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are a few gaps in alignment between Addgene's quality control sequence and the depositor's reference sequence. These mismatches are in backbone regions and should not affect function.