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pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep
(Plasmid #173899)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173899 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Total vector size (bp) 7960
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ThrRS(v1.0)
  • Insert Size (bp)
    1617

Gene/Insert 2

  • Gene/Insert name
    tRNA(v2.0)CUA
  • Insert Size (bp)
    75

Gene/Insert 3

  • Gene/Insert name
    pduX
  • Insert Size (bp)
    903

Gene/Insert 4

  • Gene/Insert name
    EF-Sep
  • Insert Size (bp)
    1299

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep was a gift from Jason W Chin (Addgene plasmid # 173899 ; http://n2t.net/addgene:173899 ; RRID:Addgene_173899)

  • For your References section:

    Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing. Zhang MS, Brunner SF, Huguenin-Dezot N, Liang AD, Schmied WH, Rogerson DT, Chin JW. Nat Methods. 2017 Jul;14(7):729-736. doi: 10.1038/nmeth.4302. Epub 2017 May 29. 10.1038/nmeth.4302 PubMed 28553966
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