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SepRS(2)/pSertRNA(B4)/EF-Sep
(Plasmid #173897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173897 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKW2
  • Total vector size (bp) 5860
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    SepRS(2)
  • Species
    Methanococcus maripaludis
  • Insert Size (bp)
    1617

Gene/Insert 2

  • Gene/Insert name
    pSertRNA
  • Insert Size (bp)
    75

Gene/Insert 3

  • Gene/Insert name
    EF-Sep
  • Insert Size (bp)
    1299

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SepRS(2)/pSertRNA(B4)/EF-Sep was a gift from Jason W Chin (Addgene plasmid # 173897 ; http://n2t.net/addgene:173897 ; RRID:Addgene_173897)

  • For your References section:

    Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog. Rogerson DT, Sachdeva A, Wang K, Haq T, Kazlauskaite A, Hancock SM, Huguenin-Dezot N, Muqit MM, Fry AM, Bayliss R, Chin JW. Nat Chem Biol. 2015 Jul;11(7):496-503. doi: 10.1038/nchembio.1823. Epub 2015 Jun 1. 10.1038/nchembio.1823 PubMed 26030730
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