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pHR-H2B-mGFP
(Plasmid #173892)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173892 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Backbone size w/o insert (bp) 9234
  • Total vector size (bp) 9612
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    378
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Promoter SFFV
  • Tag / Fusion Protein
    • mGFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-H2B-mGFP was a gift from Ron Vale (Addgene plasmid # 173892 ; http://n2t.net/addgene:173892 ; RRID:Addgene_173892)

  • For your References section:

    High-content imaging-based pooled CRISPR screens in mammalian cells. Yan X, Stuurman N, Ribeiro SA, Tanenbaum ME, Horlbeck MA, Liem CR, Jost M, Weissman JS, Vale RD. J Cell Biol. 2021 Feb 1;220(2). pii: 211696. doi: 10.1083/jcb.202008158. 10.1083/jcb.202008158 PubMed 33465779
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