pcDNA HisB TO rFez1
(Plasmid #17385)

• Depositing Lab: Stephen Goff
• Publication: Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

Backbone

• Vector backbone: pCDNA4 TO2 myc HisB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FEZ1
• Alt name: Fez1
• Alt name: fasciculation and elongation protein zeta-1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1200
• Entrez Gene: Fez1
• Tags / Fusion Proteins:
  • myc (C terminal on backbone)
  • his (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length Rat2 FEZ1 sequences, 1179 bp, was amplified using cDNA from the wild-type Rat2 fibroblasts or the mutant R3-2 line as templates. The sequences of the PCR primers for generation of C-terminal myc-His-tagged FEZ1 were as follows: forward primer, FEZ1-S1-BamHI, 5'-GCTGATGGATCCACCATGGAGGCTC CACTGGTGAGT3'; reverse primer, FEZ1-A3-XhoI, 5'-GCAACGCTCGAGCAGGTTGGACAGAGCACTTTTAAA-3'.