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pBabe-N-ZAP-Zeo
(Plasmid #17382)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17382 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-HAZ (modified pBabe vector)
  • Backbone manufacturer
    Goff Lab
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NZAP
  • Alt name
    ZAP
  • Alt name
    Zinc-finger Antiviral Protein
  • Species
    R. norvegicus (rat)
  • Mutation
    N-terminal region of ZAP (amino acids 1-254)
  • Entrez Gene
    Zc3hav1 (a.k.a. ARTD13, PARP13, Zap)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SV40pro-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-N-ZAP-Zeo was a gift from Stephen Goff (Addgene plasmid # 17382 ; http://n2t.net/addgene:17382 ; RRID:Addgene_17382)

  • For your References section:

    Inhibition of retroviral RNA production by ZAP, a CCCH-type zinc finger protein. Gao G, Guo X, Goff SP. Science. 2002 Sep 6. 297(5587):1703-6. 10.1126/science.1074276 PubMed 12215647
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