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Addgene

pSaPIPLC His
(Plasmid #173810)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173810 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli BL21-Codonplus (DE3)-RIL can be used for expression. Note that this protein purification is performed at room temperature.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Staphylococcus aureus plc
  • Alt name
    Staphylococcus aureus 1-phosphatidylinositol phosphodiesterase
  • Alt name
    Staphylococcus aureus phosphatidyl-inositol specific phospholipase C
  • Species
    Other
  • Mutation
    none
  • Promoter T7
  • Tag / Fusion Protein
    • 6XHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See UniProt ID P45723 for the mature, untagged sequence.

Also see PubMed 30148347 (bacterial PI-PLC review).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSaPIPLC His was a gift from Anne Gershenson & Mary F Roberts (Addgene plasmid # 173810 ; http://n2t.net/addgene:173810 ; RRID:Addgene_173810)

  • For your References section:

    Structure of the S. aureus PI-specific phospholipase C reveals modulation of active site access by a titratable pi-cation latched loop. Goldstein R, Cheng J, Stec B, Roberts MF. Biochemistry. 2012 Mar 27;51(12):2579-87. doi: 10.1021/bi300057q. Epub 2012 Mar 16. 10.1021/bi300057q PubMed 22390775
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