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pBtPIPLC His
(Plasmid #173808)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173808 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-23b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli BL21-Codonplus (DE3)-RIL can be used for expression. Note that this protein purification is performed at room temperature.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
  • Alt name
    Bacillus thuringiensis phosphatidyl-inositol specific phospholipase C
  • Alt name
    BtPI-PLC
  • Species
    Other
  • Mutation
    none
  • Promoter T7
  • Tag / Fusion Protein
    • 6XHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bacillus thuringiensis PI-PLC (UniProt P08954) cleaves GPI-anchored proteins off the surface of cells and can be used instead of the 97% identical, commercially available B. cereus enzyme (UniProt P14262).

Also see PubMed 30148347 (bacterial PI-PLC review).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBtPIPLC His was a gift from Anne Gershenson & Mary F Roberts (Addgene plasmid # 173808 ; http://n2t.net/addgene:173808 ; RRID:Addgene_173808)

  • For your References section:

    Fluorinated Aromatic Amino Acids Distinguish Cation-pi Interactions from Membrane Insertion. He T, Gershenson A, Eyles SJ, Lee YJ, Liu WR, Wang J, Gao J, Roberts MF. J Biol Chem. 2015 Jul 31;290(31):19334-42. doi: 10.1074/jbc.M115.668343. Epub 2015 Jun 19. 10.1074/jbc.M115.668343 PubMed 26092728
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