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Addgene

XLone-GvpB
(Plasmid #173798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173798 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    XLone
  • Backbone manufacturer
    Xiaojun Lian
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GvpB
  • Promoter TRE3G promoter
  • Tag / Fusion Protein
    • mCherry (K187N -please see depositor comments) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site Spe1 (not destroyed)
  • 5′ sequencing primer M13 fwd
  • 3′ sequencing primer M13 Rvs
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there is a single mutation K187N in mCherry. Depositor confirms this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-GvpB was a gift from Xiaojun Lian (Addgene plasmid # 173798 ; http://n2t.net/addgene:173798 ; RRID:Addgene_173798)

  • For your References section:

    A drug-selectable acoustic reporter gene system for human cell ultrasound imaging. Howells AR, Welch PJ, Kim J, Forest CR, Shi C, Lian XL. Bioeng Transl Med. 2023 Aug 2;9(2):e10584. doi: 10.1002/btm2.10584. eCollection 2024 Mar. 10.1002/btm2.10584 PubMed 38435822
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